PLAYMOL LOGO
 

Molecular news

News and updates from playMol and other molecular related sources.

Enzyme catalysis: reorganization or preorganization?


A complete understanding of how enzymes work is a hot topic within the field of computational chemistry and structural biology. One of the most controversial questions these days is the question if enzymes are completely preorganized with respect to the reaction coordinates, or if flexibility is required for reorganization to support the catalytic mechanism.

READ MORE

 

Site undergoing reconstruction


The playMol website is currently being reconstructed and improved for better user experience. One of the main focuses in this upgrade is to make the page dynamic, so that it will display properly on any device.

Note that many of the pages are not up yet, so please show patience with links leading to blank pages and dead ends.

 

News & Updates from Related Journals


Get the latest articles and updates from related journals through the incorporated RSS feed.

Currently the followin journals are included:

 

Simulation of the Tetrahedral Formation Step in Trypsin


Serine proteases, and Trypsin in particular, are some of the most thoroughly studied enzymes whith a lot of experimental data available. Trypsin is therefore a perfect candidate for theoretical computations.

In a currently ongoing project I have calculated the rate limiting step in the acylation reaction, the formation of the tetrahedral intermediate, using the MD/FEP/EVB technique. The activation energy (transition state, TS), Δ G, obtained from the calculations are around 19.5 kcal/mol. This is in excellent agreement with the substrate dependent barrier that typically is 15 - 20 kcal/mol.

The reaction profile is illustrated below where RS is the reactant state, TS the transition state and TET is the tetrahedral intermediate.

Reaction profile for the thetrahedral intermediate formation step in bovine trypsin.

A animation with 100 ps of the trajectory around the transition state illustrates the system changing from the reactant state to the tetrahedral intermediate. Notice that water molecules (~5000) have been hidden in the animation for better visibility. Only the counter ions (Cl-) are visible.

View the animation here!